SeqBuster is a web-based bioinformatic tool offering a custom analysis of deep sequencing data at different levels, with special emphasis on the analysis of miRNA variants or isomiRs.SeqBuster includes a stand-alone version that permits the annotation against any custom database installed in the local machine independently of the web server tool. This offers a pre-analysis that is not restricted to the databases stored in the web server, overcoming the limitations in the storage capacities detected in other web-based bioinformatic tools. The R environment in which the different analysis packages have been developed permits the incorporation and/or modification of different types of analysis, which may be focused not only on small non-coding RNAs, but also on any type of sequence generated in large-scale sequencing strategies. SeqBuster is highly versatile offering a wide range of options both in the pre-analysis for annotation purposes and in the different module analysis for data manipulation.
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SeqBuster is developed by the Genes and Diseases Group at the Centre for Genomic Regulation (Sirocco Partner 14). It has been developed and was presented to the Sirocco at the Sirocco Conference in 2009.